cxcl12 staining  (R&D Systems)

Journal: Tumour Biology

Article Title: CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis

doi: 10.1007/s13277-014-1816-1

Figure Lengend Snippet: CXCL12 and CXCR4 expression in 182 patients with breast cancer

Article Snippet: Immunohistochemical staining for CXCL12 (sc-28876, 1:100, Santa Cruz, USA) and CXCR4 (sc-53534, 1:500, Santa Cruz, USA) was performed using UltraSensitiveTM S-P kits (Maixin-Bio, China) according to the manufacturer's instructions and using the reagent supplied with the kit.

Techniques: Expressing

Journal: Tumour Biology

Article Title: CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis

doi: 10.1007/s13277-014-1816-1

Figure Lengend Snippet: Co-expression of CXCL12 and CXCR4 in 182 patients with breast cancer

Article Snippet: Immunohistochemical staining for CXCL12 (sc-28876, 1:100, Santa Cruz, USA) and CXCR4 (sc-53534, 1:500, Santa Cruz, USA) was performed using UltraSensitiveTM S-P kits (Maixin-Bio, China) according to the manufacturer's instructions and using the reagent supplied with the kit.

Techniques:

Journal: Tumour Biology

Article Title: CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis

doi: 10.1007/s13277-014-1816-1

Figure Lengend Snippet: The expression patterns of CXCL12 and CXCR4 in breast cancer cell lines. Total RNA and proteins were extracted from wild-type MCF-7, wild-type MDA-MB-435s, wild-type MDA-MB-231, MDA-MB-231–CXCL12 and MDA-MB-231–ZsGreen1. Then they were subjected to RT-PCR and western blot analysis, respectively, for CXCL12 and CXCR4. Both mRNA ( a ) and protein ( b ) expression of CXCR4 and very weak expression of CXCL12 were observed consistently in MDA-MB-231. CXCR4 and CXCL12 mRNA ( a ) and protein ( b ) expression was found in MDA-MB-435s. MCF-7 has very weak CXCR4 and CXCL12 mRNA and protein expression ( a and b ). So we picked MDA-MB-231 to be transfected with CXCL12. CXCL12 mRNA ( a ) and protein ( b ) expression was observed in MDA-MB-231–CXCL12 cells, but very weak in MDA-MB-231–ZsGreen1 or wild-type MDA-MB-231 cells. It is indicated that the stable CXCL12 transfection in MDA-MB-231 was successfully constructed

Article Snippet: Immunohistochemical staining for CXCL12 (sc-28876, 1:100, Santa Cruz, USA) and CXCR4 (sc-53534, 1:500, Santa Cruz, USA) was performed using UltraSensitiveTM S-P kits (Maixin-Bio, China) according to the manufacturer's instructions and using the reagent supplied with the kit.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection, Construct

Journal: Tumour Biology

Article Title: CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis

doi: 10.1007/s13277-014-1816-1

Figure Lengend Snippet: The proliferation curve of MDA-MB-231–CXCL12 and MDA-MB-231–ZsGreen1 was constructed by plotting absorbance against time using the MTT method. Values represent the mean ± SD of five independent experiments. The cell proliferation rate of MDA-MB-231–CXCL12 cells was markedly lower than that of MDA-MB-231–ZsGreen1 or wild-type MDA-MB-231 cells at 48 h, 60 h, and 48 h ( p < 0.05). * indicates a statistically significant difference between MDA-MB-231–CXCL12 group and MDA-MB-231–ZsGreen1 group ( p < 0.05)

Article Snippet: Immunohistochemical staining for CXCL12 (sc-28876, 1:100, Santa Cruz, USA) and CXCR4 (sc-53534, 1:500, Santa Cruz, USA) was performed using UltraSensitiveTM S-P kits (Maixin-Bio, China) according to the manufacturer's instructions and using the reagent supplied with the kit.

Techniques: Construct

Journal: Tumour Biology

Article Title: CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis

doi: 10.1007/s13277-014-1816-1

Figure Lengend Snippet: The CXCL12 transfection increased the invasive ability of breast cancer cell MDA-MB-231. Matrigel invasion assay showed that the invasive ability of the wild-type MDA-MB-231 ( a ), MDA-MB-231–ZsGreen1 ( b ) and MDA-MB-231–CXCL12 ( c ) at 48 h after transfection ( d ) Number of cells invading onto the lower surfaces of the filter was counted. Data represented the mean±SD of three independent experiments, * p <0.05 vs. wild-type MDA-MB-231 group, # p <0.05 vs. MDA-MB-231–ZsGreen1 group

Article Snippet: Immunohistochemical staining for CXCL12 (sc-28876, 1:100, Santa Cruz, USA) and CXCR4 (sc-53534, 1:500, Santa Cruz, USA) was performed using UltraSensitiveTM S-P kits (Maixin-Bio, China) according to the manufacturer's instructions and using the reagent supplied with the kit.

Techniques: Transfection, Invasion Assay